Protein-Based Diagnostics Primer
*This microcourse includes optional CPE Credits and is powered by Biotech Primer, a trusted leader in life science training that simplifies complex topics for non-scientists.
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Optional CPE Credit: 1.0
Overview:
Protein-Based Diagnostics Primer is an extensive tutorial on protein-based diagnostics commonly used in disease detection in the research and clinic setting. This engaging class covers various tools, including immunoassays, multiplexed assays, lateral flow assays, and chromatography. Get ready to explore the science of ELISA and bead immunoassays, two quantitative diagnostics that measure multiple proteins simultaneously. This course also takes a closer look at Lateral Flow Assay (LFA) technology, a rapid detection diagnostic for target-specific biomarkers. To round out the agenda, this primer ends with an in-depth exploration of chromatography technologies used for protein separation, purification, and analysis.
- Section 1: Defining Protein-Based Diagnostics: Introduces protein-based diagnostics by explaining the importance of biomarkers in diagnostics. A biomarker is a measurable molecule indicative of disease.
- Section 2: Antibody Technology: Explains the importance of antibody structure and how that structure contributes to its function. Antibody structure and function are exploited by the medical device industry for diagnostic use.
- Section 3: Enzyme-Linked Immunosorbent Assays (ELISA) Technology: Demonstrates the technology and how to interpret results of the widely-used screening test known as an ELISA.
- Session 4: Bead Immunoassay Technology: Extends your ELISA knowledge by showing how ELISA technology can be adapted into more high throughput techniques, such as multiplexed bead assays.
- Session 5: Lateral Flow Assay Technology: Explains how lateral flow immunochromatographic assay works, its applications and how to interpret its results.
- Section 6: Chromatography Technology: Describes the applications of column chromatography in diagnostics and how to interpret its results.
By the end of the course, participants will be able to:
- Discuss what makes antibodies uniquely appropriate for use in protein diagnostics.
- List examples of protein-based diagnostics and state their uses.
- Describe how biomarkers are used in diagnostics.
- Summarize the fundamental science of protein-based diagnostics.
- Analyze and interpret the findings from ELISA, bead immunoassays, lateral flow assays, and chromatography diagnostics.
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